HAMNO
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 555146

CAS#: 138736-73-9

Description: HAMNO, also known as NSC-111847, is a potent and selective inhibitor of replication protein A (RPA) interactions with proteins involved in the replication stress response.


Chemical Structure

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HAMNO
CAS# 138736-73-9

Theoretical Analysis

MedKoo Cat#: 555146
Name: HAMNO
CAS#: 138736-73-9
Chemical Formula: C17H13NO2
Exact Mass: 263.09
Molecular Weight: 263.296
Elemental Analysis: C, 77.55; H, 4.98; N, 5.32; O, 12.15

Price and Availability

Size Price Availability Quantity
1g USD 2250
2g USD 3950
500mg USD 1650
200mg USD 950
100mg USD 450 2 Weeks
50mg USD 250 2 Weeks
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Synonym: HAMNO; CID 6335338, MLS000737724, NSC111847, NSC-111847; NSC111847.

IUPAC/Chemical Name: (1Z)-1-[(2-Hydroxyanilino)methylidene]naphthalen-2-one

InChi Key: NADCEWZYITXLKJ-KAMYIIQDSA-N

InChi Code: InChI=1S/C17H13NO2/c19-16-10-9-12-5-1-2-6-13(12)14(16)11-18-15-7-3-4-8-17(15)20/h1-11,18,20H/b14-11-

SMILES Code: O=C(C=CC1=C/2C=CC=C1)C2=C/NC3=CC=CC=C3O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Product Data:
Biological target: HAMNO is a novel protein interaction inhibitor of replication protein A (RPA).
In vitro activity: These data suggest that HAMNO selectively increased γ-H2AX staining in S-phase, indicative of increased replicative stress. Reference: Cancer Res. 2014 Sep 15;74(18):5165-72. https://pubmed.ncbi.nlm.nih.gov/25070753/
In vivo activity: Ser33 of RPA32, an ATR substrate, is highly phosphorylated after two hours of treatment with 20 μM of etoposide, which was reduced with the addition of 2 μM HAMNO, and was nearly absent at higher concentrations, demonstrating an in vivo effect of HAMNO as an inhibitor of RPA32 phosphorylation by ATR in mice. Reference: Cancer Res. 2014 Sep 15;74(18):5165-72. https://pubmed.ncbi.nlm.nih.gov/25070753/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 30.0 113.94
DMSO 30.0 113.94
DMSO:PBS (pH 7.2) (1:30) 0.3 0.95

Preparing Stock Solutions

The following data is based on the product molecular weight 263.296000000000000000000000000000 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Glanzer JG, Liu S, Wang L, Mosel A, Peng A, Oakley GG. RPA inhibition increases replication stress and suppresses tumor growth. Cancer Res. 2014 Sep 15;74(18):5165-72. doi: 10.1158/0008-5472.CAN-14-0306. Epub 2014 Jul 28. PMID: 25070753; PMCID: PMC4201622.
In vitro protocol: 1. Glanzer JG, Liu S, Wang L, Mosel A, Peng A, Oakley GG. RPA inhibition increases replication stress and suppresses tumor growth. Cancer Res. 2014 Sep 15;74(18):5165-72. doi: 10.1158/0008-5472.CAN-14-0306. Epub 2014 Jul 28. PMID: 25070753; PMCID: PMC4201622.
In vivo protocol: 1. Glanzer JG, Liu S, Wang L, Mosel A, Peng A, Oakley GG. RPA inhibition increases replication stress and suppresses tumor growth. Cancer Res. 2014 Sep 15;74(18):5165-72. doi: 10.1158/0008-5472.CAN-14-0306. Epub 2014 Jul 28. PMID: 25070753; PMCID: PMC4201622.

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