WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 561669

CAS#: 210344-95-9

Description: Z-DEVD-fmk is a cell-permeable, irreversible inhibitor of caspase-3. Caspase-3 is a cysteinyl aspartate-specific protease which plays a central role in apoptosis.

Chemical Structure

CAS# 210344-95-9

Theoretical Analysis

MedKoo Cat#: 561669
Name: Z-DEVD-fmk
CAS#: 210344-95-9
Chemical Formula: C30H41FN4O12
Exact Mass: 668.2705
Molecular Weight: 668.67
Elemental Analysis: C, 53.89; H, 6.18; F, 2.84; N, 8.38; O, 28.71

Price and Availability

Size Price Availability Quantity
25.0mg USD 950.0 2 Weeks
50.0mg USD 1650.0 2 Weeks
100.0mg USD 2650.0 2 Weeks
200.0mg USD 4650.0 2 Weeks
500.0mg USD 6650.0 2 Weeks
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Synonym: Z-DEVD-fmk; Z DEVD fmk; ZDEVDfmk;

IUPAC/Chemical Name: methyl (5S,8S,11S,14S)-14-(2-fluoroacetyl)-11-isopropyl-5-(2-methoxy-2-oxoethyl)-8-(3-methoxy-3-oxopropyl)-3,6,9,12-tetraoxo-1-phenyl-2-oxa-4,7,10,13-tetraazahexadecan-16-oate


InChi Code: InChI=1S/C30H41FN4O12/c1-17(2)26(29(42)33-20(22(36)15-31)13-24(38)45-4)35-27(40)19(11-12-23(37)44-3)32-28(41)21(14-25(39)46-5)34-30(43)47-16-18-9-7-6-8-10-18/h6-10,17,19-21,26H,11-16H2,1-5H3,(H,32,41)(H,33,42)(H,34,43)(H,35,40)/t19-,20-,21-,26-/m0/s1

SMILES Code: O=C(OC)CC[C@H](NC([C@@H](NC(OCC1=CC=CC=C1)=O)CC(OC)=O)=O)C(N[C@@H](C(C)C)C(N[C@H](C(CF)=O)CC(OC)=O)=O)=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 668.67 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Sridhar V, Arepalli SK, Velatooru LR, Venkateswara Rao J, Kavin Kennedy P, Narsaiah B. In vitro apoptotic mechanism of a novel synthetic Quinazolinyl derivative: Induces caspase-dependent intrinsic pathway on THP-1, leukemia cell line. Chem Biol Interact. 2017 Dec 7. pii: S0009-2797(17)30715-9. doi: 10.1016/j.cbi.2017.12.015. [Epub ahead of print] PubMed PMID: 29225136.

2: Yemisci M, Caban S, Fernandez-Megia E, Capan Y, Couvreur P, Dalkara T. Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides. Methods Mol Biol. 2018;1727:443-454. doi: 10.1007/978-1-4939-7571-6_36. PubMed PMID: 29222804.

3: Furukawa Y, Takasu A, Yura Y. Role of autophagy in oncolytic herpes simplex virus type 1-induced cell death in squamous cell carcinoma cells. Cancer Gene Ther. 2017 Sep;24(9):393-400. doi: 10.1038/cgt.2017.33. Epub 2017 Oct 6. PubMed PMID: 28984290.

4: Zhou P, Dong XX, Tang P. [Sanggenon C induces apoptosis of prostate cancer PC3 cells by activating caspase 3 and caspase 9 pathways]. Nan Fang Yi Ke Da Xue Xue Bao. 2017 Sep 20;37(9):1206-1210. Chinese. PubMed PMID: 28951363.

5: Yang Y, Du J, Liu F, Wang X, Li X, Li Y. Role of caspase-3/E-cadherin in helicobacter pylori-induced apoptosis of gastric epithelial cells. Oncotarget. 2017 Jul 22;8(35):59204-59216. doi: 10.18632/oncotarget.19471. eCollection 2017 Aug 29. PubMed PMID: 28938629; PubMed Central PMCID: PMC5601725.

6: Pyrshev KA, Yesylevskyy SO, Mély Y, Demchenko AP, Klymchenko AS. Caspase-3 activation decreases lipid order in the outer plasma membrane leaflet during apoptosis: A fluorescent probe study. Biochim Biophys Acta. 2017 Oct;1859(10):2123-2132. doi: 10.1016/j.bbamem.2017.08.002. Epub 2017 Aug 5. PubMed PMID: 28784460.

7: Shang HS, Shih YL, Lu TJ, Lee CH, Hsueh SC, Chou YC, Lu HF, Liao NC, Chung JG. Benzyl isothiocyanate (BITC) induces apoptosis of GBM 8401 human brain glioblastoma multiforms cells via activation of caspase-8/Bid and the reactive oxygen species-dependent mitochondrial pathway. Environ Toxicol. 2016 Dec;31(12):1751-1760. doi: 10.1002/tox.22177. Epub 2015 Sep 7. PubMed PMID: 28675694.

8: Maimaitili A, Shu Z, Cheng X, Kaheerman K, Sikandeer A, Li W. Arctigenin, a natural lignan compound, induces G(0)/G(1) cell cycle arrest and apoptosis in human glioma cells. Oncol Lett. 2017 Feb;13(2):1007-1013. doi: 10.3892/ol.2016.5474. Epub 2016 Dec 8. PubMed PMID: 28356992; PubMed Central PMCID: PMC5351207.

9: Chen CH, Chen MF, Huang SJ, Huang CY, Wang HK, Hsieh WC, Huang CH, Liu LF, Shiu LY. Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells. Am J Chin Med. 2017;45(2):351-368. doi: 10.1142/S0192415X17500227. Epub 2017 Feb 23. PubMed PMID: 28231747.

10: Zhu H, Tan L, Li Y, Li J, Qiu M, Li L, Zhang M, Liang M, Li A. Increased Apoptosis in the Paraventricular Nucleus Mediated by AT1R/Ras/ERK1/2 Signaling Results in Sympathetic Hyperactivity and Renovascular Hypertension in Rats after Kidney Injury. Front Physiol. 2017 Feb 2;8:41. doi: 10.3389/fphys.2017.00041. eCollection 2017. PubMed PMID: 28210225; PubMed Central PMCID: PMC5288364.

11: Chen M, Cai F, Zha D, Wang X, Zhang W, He Y, Huang Q, Zhuang H, Hua ZC. Astragalin-induced cell death is caspase-dependent and enhances the susceptibility of lung cancer cells to tumor necrosis factor by inhibiting the NF-кB pathway. Oncotarget. 2017 Apr 18;8(16):26941-26958. doi: 10.18632/oncotarget.15264. PubMed PMID: 28199969; PubMed Central PMCID: PMC5432309.

12: Chen RT, Jiao P, Liu Z, Lu Y, Xin HH, Zhang DP, Miao YG. Role of BmDredd during Apoptosis of Silk Gland in Silkworm, Bombyx mori. PLoS One. 2017 Jan 9;12(1):e0169404. doi: 10.1371/journal.pone.0169404. eCollection 2017. PubMed PMID: 28068357; PubMed Central PMCID: PMC5222620.

13: Saini A, Sharples AP, Al-Shanti N, Stewart CE. Omega-3 fatty acid EPA improves regenerative capacity of mouse skeletal muscle cells exposed to saturated fat and inflammation. Biogerontology. 2017 Feb;18(1):109-129. doi: 10.1007/s10522-016-9667-3. Epub 2016 Nov 18. PubMed PMID: 27864687; PubMed Central PMCID: PMC5288450.

14: Rotschafer SE, Allen-Sharpley MR, Cramer KS. Axonal Cleaved Caspase-3 Regulates Axon Targeting and Morphogenesis in the Developing Auditory Brainstem. Front Neural Circuits. 2016 Oct 24;10:84. eCollection 2016. PubMed PMID: 27822180; PubMed Central PMCID: PMC5075536.

15: Downs RM, Hughes MA, Kinsey ST, Johnson MC, Baumgarner BL. Inhibiting c-Jun N-terminal kinase partially attenuates caffeine-dependent cell death without alleviating the caffeine-induced reduction in mitochondrial respiration in C2C12 skeletal myotubes. Biochem Biophys Res Commun. 2016 Nov 4;480(1):61-68. doi: 10.1016/j.bbrc.2016.10.008. Epub 2016 Oct 4. PubMed PMID: 27717822; PubMed Central PMCID: PMC5580259.

16: Park EY, Kim JI, Leem DG, Shin JS, Kim KT, Choi SY, Lee MH, Choi JH, Lee YS, Lee KT. Resveratrol analogue (E)-8-acetoxy-2-[2-(3,4-diacetoxyphenyl)ethenyl]-quinazoline induces apoptosis via Fas-mediated pathway in HL-60 human leukemia cells. Oncol Rep. 2016 Dec;36(6):3577-3587. doi: 10.3892/or.2016.5168. Epub 2016 Oct 13. PubMed PMID: 27748905.

17: Uto T, Tung NH, Thongjankaew P, Lhieochaiphant S, Shoyama Y. Kayeassamin A Isolated from the Flower of Mammea siamensis Triggers Apoptosis by Activating Caspase-3/-8 in HL-60 Human Leukemia Cells. Pharmacognosy Res. 2016 Oct-Dec;8(4):244-248. PubMed PMID: 27695262; PubMed Central PMCID: PMC5004513.

18: Chen MF, Huang SJ, Huang CC, Liu PS, Lin KI, Liu CW, Hsieh WC, Shiu LY, Chen CH. Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells. BMC Cancer. 2016 Jul 26;16:532. doi: 10.1186/s12885-016-2599-0. PubMed PMID: 27461108; PubMed Central PMCID: PMC4962421.

19: Panda PK, Behera B, Meher BR, Das DN, Mukhopadhyay S, Sinha N, Naik PP, Roy B, Das J, Paul S, Maiti TK, Agarwal R, Bhutia SK. Abrus Agglutinin, a type II ribosome inactivating protein inhibits Akt/PH domain to induce endoplasmic reticulum stress mediated autophagy-dependent cell death. Mol Carcinog. 2017 Feb;56(2):389-401. doi: 10.1002/mc.22502. Epub 2016 May 31. PubMed PMID: 27182794.

20: Yin P, Jia J, Li J, Song Y, Zhang Y, Chen F. ABT-737, a Bcl-2 Selective Inhibitor, and Chloroquine Synergistically Kill Renal Cancer Cells. Oncol Res. 2016;24(1):65-72. doi: 10.3727/096504016X14587366983838. PubMed PMID: 27178823.