WARNING: This product is for research use only, not for human or veterinary use.
MedKoo CAT#: 530993
CAS#: 120241-79-4
Description: 3-TYP is an inhibitor of SIRT3.
MedKoo Cat#: 530993
Name: 3-TYP
CAS#: 120241-79-4
Chemical Formula: C7H6N4
Exact Mass: 146.0592
Molecular Weight: 146.15
Elemental Analysis: C, 57.53; H, 4.14; N, 38.34
Synonym: 3-TYP; 3TYP; 3 TYP
IUPAC/Chemical Name: 3-(1H-1,2,3-triazol-4-yl) pyridine
InChi Key: VYXFEFOIYPNBFK-UHFFFAOYSA-N
InChi Code: InChI=1S/C7H6N4/c1-2-6(4-8-3-1)7-5-9-11-10-7/h1-5H,(H,9,10,11)
SMILES Code: C1(C2=CNN=N2)=CC=CN=C1
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
| Biological target: | 3-TYP is a selective SIRT3 inhibitor, with an IC50 of 16 nM, more potent over SIRT1 (IC50=88 nM), SIRT2 (IC50=92 nM). |
| In vitro activity: | 3-TYP inhibits melatonin-enhanced SIRT3 activity but does not affect SIRT3 protein expression. 3-TYP pretreatment reverses the protective effects of melatonin on cadmium (Cd)-induced mitochondrial-derived O2•− production and autophagic cell death. 3-TYP significantly attenuates melatonin-induced increases in deacetylated-SOD2 expression and SOD2 activity in HepG2 cells exposed to Cd. Reference: Autophagy. 2015;11(7):1037-51. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26120888/ |
| In vivo activity: | 3-TYP (50 mg/kg, i.p.) does not significantly influence the LVEF, LVFS, infarct size, serum LDH levels, apoptosis, and oxidative stress compared with those of the Sham group. Moreover, 3-TYP has little effect on gp91phox, Nrf2, NQO 1, Bax, Bcl-2, Caspase-3, and cleaved Caspase-3 expression levels, compared with the Sham group. 3-TYP significantly decreases SIRT3 activity and increases the acetylation of SOD2 compared with that in the control group, without influencing SIRT3 expression. 3-TYP attenuates the cardioprotective effects of melatonin by decreasing the LVEF and LVFS after 24 hour of reperfusion. 3-TYP also increases the infarct size, serum LDH levels, and apoptotic ratio compared with those in the IR+Mel group. Reference: J Pineal Res. 2017 Sep;63(2). https://doi.org/10.1111/jpi.12419 |
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 29.0 | 198.43 | |
| Ethanol | 29.0 | 198.43 |
The following data is based on the product molecular weight 146.15 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| In vitro protocol: | 1. Pi H, Xu S, Reiter RJ, Guo P, Zhang L, Li Y, Li M, Cao Z, Tian L, Xie J, Zhang R, He M, Lu Y, Liu C, Duan W, Yu Z, Zhou Z. SIRT3-SOD2-mROS-dependent autophagy in cadmium-induced hepatotoxicity and salvage by melatonin. Autophagy. 2015;11(7):1037-51. doi: 10.1080/15548627.2015.1052208. PMID: 26120888; PMCID: PMC4590599. |
| In vivo protocol: | 1. Zhai M, Li B, Duan W, Jing L, Zhang B, Zhang M, Yu L, Liu Z, Yu B, Ren K, Gao E, Yang Y, Liang H, Jin Z, Yu S. Melatonin ameliorates myocardial ischemia reperfusion injury through SIRT3-dependent regulation of oxidative stress and apoptosis. J Pineal Res. 2017 Sep;63(2). doi: 10.1111/jpi.12419. Epub 2017 Jun 20. PMID: 28500761. |
1: Zhai M, Li B, Duan W, Jing L, Zhang B, Zhang M, Yu L, Liu Z, Yu B, Ren K, Gao
E, Yang Y, Liang H, Jin Z, Yu S. Melatonin ameliorates myocardial ischemia
reperfusion injury through SIRT3-dependent regulation of oxidative stress and
apoptosis. J Pineal Res. 2017 Sep;63(2). doi: 10.1111/jpi.12419. Epub 2017 Jun
20. PubMed PMID: 28500761.
2: Zeng Z, Yang Y, Dai X, Xu S, Li T, Zhang Q, Zhao KS, Chen Z. Polydatin
ameliorates injury to the small intestine induced by hemorrhagic shock via SIRT3
activation-mediated mitochondrial protection. Expert Opin Ther Targets. 2016
Jun;20(6):645-52. doi: 10.1080/14728222.2016.1177023. Epub 2016 Apr 22. PubMed
PMID: 27067422.
3: Pi H, Xu S, Reiter RJ, Guo P, Zhang L, Li Y, Li M, Cao Z, Tian L, Xie J, Zhang
R, He M, Lu Y, Liu C, Duan W, Yu Z, Zhou Z. SIRT3-SOD2-mROS-dependent autophagy
in cadmium-induced hepatotoxicity and salvage by melatonin. Autophagy.
2015;11(7):1037-51. doi: 10.1080/15548627.2015.1052208. PubMed PMID: 26120888;
PubMed Central PMCID: PMC4590599.
Sirtuins are a family of highly evolutionarily conserved nicotinamide adenine nucleotide-dependent histone deacetylases. Sirtuin-3 (SIRT3) is a member of the sirtuin family that is localized primarily to the mitochondria and protects against oxidative stress-related diseases, including myocardial ischemia/reperfusion (MI/R) injury. Melatonin has a favorable effect in ameliorating MI/R injury. We hypothesized that melatonin protects against MI/R injury by activating the SIRT3 signaling pathway.
