IBMX
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    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 561233

CAS#: 28822-58-4

Description: IBMX is a competitive nonselective phosphodiesterase inhibitor. IBMX raises intracellular cAMP, activates PKA, inhibits TNFα and leukotriene synthesis, and reduces inflammation and innate immunity. IBMX is also a nonselective adenosine receptor antagonist.


Chemical Structure

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IBMX
CAS# 28822-58-4

Theoretical Analysis

MedKoo Cat#: 561233
Name: IBMX
CAS#: 28822-58-4
Chemical Formula: C10H14N4O2
Exact Mass: 222.11
Molecular Weight: 222.248
Elemental Analysis: C, 54.04; H, 6.35; N, 25.21; O, 14.40

Price and Availability

Size Price Availability Quantity
250mg USD 350 2 Weeks
1g USD 750 2 Weeks
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Synonym: IBMX

IUPAC/Chemical Name: 3-Isobutyl-1-methylxanthine

InChi Key: APIXJSLKIYYUKG-UHFFFAOYSA-N

InChi Code: InChI=1S/C10H14N4O2/c1-6(2)4-14-8-7(11-5-12-8)9(15)13(3)10(14)16/h5-6H,4H2,1-3H3,(H,11,12)

SMILES Code: O=C(N1C)N(CC(C)C)C2=C(NC=N2)C1=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: IBMX is a broad-spectrum phosphodiesterase (PDE) inhibitor, with IC50s of 6.5, 26.3 and 31.7 μM for PDE3, PDE4 and PDE5, respectively.
In vitro activity: Subsequently, B16/F10 melanoma cells were treated with IBMX to analyze the effect of ESB on melanin production. As shown in Figure 4B, the level of melanin content increased to 316.85% when treated with IBMX. On the other hand, the ESB treated group (108.60%) at 10 μg/mL concentration showed significantly decreased melanin contents compared to the IBMX treated group, and similar melanin contents compared to the arbutin group (101.79%) as a positive control. Reference: Nutrients. 2020 Mar 20;12(3):832. https://pubmed.ncbi.nlm.nih.gov/32245029/
In vivo activity: There was a subsequent decrease in PDCD4 protein (0.75 ± 0.05 relative band intensity) in oocytes under IBMX inhibition at 24 h compared to control (1.2 ± 0.04 relative band intensity; P = 0.03; Fig. 2b). This suggests that IBMX inhibition of GVBD increased the abundance of MIR21 in the oocyte hastening the MIR21-driven decrease in PDCD4 abundance. Reference: Reprod Biol Endocrinol. 2020 May 11;18(1):39. https://pubmed.ncbi.nlm.nih.gov/32393269/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 5.0 22.50
DMSO 25.6 114.99
Ethanol 6.1 27.31

Preparing Stock Solutions

The following data is based on the product molecular weight 222.25 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Han HJ, Park SK, Kang JY, Kim JM, Yoo SK, Heo HJ. Anti-Melanogenic Effect of Ethanolic Extract of Sorghum bicolor on IBMX-Induced Melanogenesis in B16/F10 Melanoma Cells. Nutrients. 2020 Mar 20;12(3):832. doi: 10.3390/nu12030832. PMID: 32245029; PMCID: PMC7146600. 2. Wei Y, Liao Y, Zavilowitz B, Ren J, Liu W, Chan P, Rohatgi R, Estilo G, Jackson EK, Wang WH, Satlin LM. Angiotensin II type 2 receptor regulates ROMK-like K⁺ channel activity in the renal cortical collecting duct during high dietary K⁺ adaptation. Am J Physiol Renal Physiol. 2014 Oct 1;307(7):F833-43. doi: 10.1152/ajprenal.00141.2014. Epub 2014 Aug 6. PMID: 25100281; PMCID: PMC4187043. 3. Hale BJ, Li Y, Adur MK, Ross JW. Inhibition of germinal vesicle breakdown using IBMX increases microRNA-21 in the porcine oocyte. Reprod Biol Endocrinol. 2020 May 11;18(1):39. doi: 10.1186/s12958-020-00603-1. PMID: 32393269; PMCID: PMC7212575. 4. Candia OA, Kong CW, Alvarez LJ. IBMX-elicited inhibition of water permeability in the isolated rabbit conjunctival epithelium. Exp Eye Res. 2008 Mar;86(3):480-91. doi: 10.1016/j.exer.2007.11.021. Epub 2007 Dec 23. PMID: 18234193; PMCID: PMC2288740.
In vitro protocol: 1. Han HJ, Park SK, Kang JY, Kim JM, Yoo SK, Heo HJ. Anti-Melanogenic Effect of Ethanolic Extract of Sorghum bicolor on IBMX-Induced Melanogenesis in B16/F10 Melanoma Cells. Nutrients. 2020 Mar 20;12(3):832. doi: 10.3390/nu12030832. PMID: 32245029; PMCID: PMC7146600. 2. Wei Y, Liao Y, Zavilowitz B, Ren J, Liu W, Chan P, Rohatgi R, Estilo G, Jackson EK, Wang WH, Satlin LM. Angiotensin II type 2 receptor regulates ROMK-like K⁺ channel activity in the renal cortical collecting duct during high dietary K⁺ adaptation. Am J Physiol Renal Physiol. 2014 Oct 1;307(7):F833-43. doi: 10.1152/ajprenal.00141.2014. Epub 2014 Aug 6. PMID: 25100281; PMCID: PMC4187043.
In vivo protocol: 1. Hale BJ, Li Y, Adur MK, Ross JW. Inhibition of germinal vesicle breakdown using IBMX increases microRNA-21 in the porcine oocyte. Reprod Biol Endocrinol. 2020 May 11;18(1):39. doi: 10.1186/s12958-020-00603-1. PMID: 32393269; PMCID: PMC7212575. 2. Candia OA, Kong CW, Alvarez LJ. IBMX-elicited inhibition of water permeability in the isolated rabbit conjunctival epithelium. Exp Eye Res. 2008 Mar;86(3):480-91. doi: 10.1016/j.exer.2007.11.021. Epub 2007 Dec 23. PMID: 18234193; PMCID: PMC2288740.

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