H8 dihydrochloride
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MedKoo CAT#: 540190

CAS#: 113276-94-1

Description: H8 dihydrochloride is a PKA inhibitor that modulates Ca 2+ signaling.


Chemical Structure

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H8 dihydrochloride
CAS# 113276-94-1

Theoretical Analysis

MedKoo Cat#: 540190
Name: H8 dihydrochloride
CAS#: 113276-94-1
Chemical Formula: C12H17Cl2N3O2S
Exact Mass: 0.00
Molecular Weight: 338.247
Elemental Analysis: C, 42.61; H, 5.07; Cl, 20.96; N, 12.42; O, 9.46; S, 9.48

Price and Availability

Size Price Availability Quantity
10mg USD 295 2 Weeks
50mg USD 650 2 Weeks
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Synonym: H8; H8 dihydrochloride.

IUPAC/Chemical Name: N-(2-(methylamino)ethyl)isoquinoline-5-sulfonamide dihydrochloride

InChi Key: RJJLZYZEVNCZIW-UHFFFAOYSA-N

InChi Code: InChI=1S/C12H15N3O2S.2ClH/c1-13-7-8-15-18(16,17)12-4-2-3-10-9-14-6-5-11(10)12;;/h2-6,9,13,15H,7-8H2,1H3;2*1H

SMILES Code: O=S(C1=CC=CC2=C1C=CN=C2)(NCCNC)=O.[H]Cl.[H]Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: A potent, nonspecific kinase inhibitor.
In vitro activity: H-8 was the most active of the inhibitors in this series and inhibited more markedly cyclic nucleotide dependent protein kinases, than other kinases, while the derivative with the sulfonylpiperazine residue (H-7) was the most potent in inhibiting protein kinase C. Reference: Biochemistry. 1984 Oct 9;23(21):5036-41. https://pubmed.ncbi.nlm.nih.gov/6238627/
In vivo activity: TBD

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 20.0 59.13
DMSO 20.0 59.13
PBS (pH 7.2) 10.0 29.56

Preparing Stock Solutions

The following data is based on the product molecular weight 338.25 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Hidaka H, Inagaki M, Kawamoto S, Sasaki Y. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry. 1984 Oct 9;23(21):5036-41. doi: 10.1021/bi00316a032. PMID: 6238627.
In vitro protocol: 1. Hidaka H, Inagaki M, Kawamoto S, Sasaki Y. Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry. 1984 Oct 9;23(21):5036-41. doi: 10.1021/bi00316a032. PMID: 6238627.
In vivo protocol: TBD

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