Hematein
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MedKoo CAT#: 531025

CAS#: 475-25-2

Description: Hematein, also known as Haematein, is an oxidized derivative of Hematoxylin (Haematoxylin) used in staining the staining of cell nuclei in animal tissue. Hematein also can be used in the staining of myelin sheaths and nerve fibers. This product has low purity ( the estimated HPLC purity is ~ 50%).


Chemical Structure

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Hematein
CAS# 475-25-2

Theoretical Analysis

MedKoo Cat#: 531025
Name: Hematein
CAS#: 475-25-2
Chemical Formula: C16H12O6
Exact Mass: 300.0634
Molecular Weight: 300.266
Elemental Analysis: C, 64.00; H, 4.03; O, 31.97

Price and Availability

Size Price Availability Quantity
5.0g USD 190.0 Same day
10.0g USD 350.0 Same day
25.0g USD 650.0 Same day
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Related CAS #: 475-25-2 (Hematein)   517-28-2 (Hematoxylin)  

Synonym: Hematein, haematein, Hematoxylin, Haematoxylin, AI3-63013, AI3 63013, AI363013, NSC 7333, NSC-7333, NSC7333,

IUPAC/Chemical Name: 3,4,6a,10-tetrahydroxy-6a,7-dihydroindeno[2,1-c]chromen-9(6H)-one

InChi Key: HLUCICHZHWJHLL-UHFFFAOYSA-N

InChi Code: InChI=1S/C16H12O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,17,19-21H,5-6H2

SMILES Code: O=C1C=C2CC3(O)C(C4=CC=C(O)C(O)=C4OC3)=C2C=C1O

Appearance: Light brown to brown solid powder.

Purity: CORRESPONDS TO STANDARD (the estimated HPLC purity is ~ 50%)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Certificate of Analysis:

Safety Data Sheet (SDS):

Preparing Stock Solutions

The following data is based on the product molecular weight 300.266 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Panayotacopoulou MT, Boukis D, Issidorides MR. Localization of phospholipids in human catecholamine neurons with Baker's acid haematein. J Neurocytol. 1989 Oct;18(5):593-7. PubMed PMID: 2482342.

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7: KROON DB. CERTAIN CELLS IN THE HYPOTHALAMIC NEUROSECRETORY NUCLEI WHICH ARE STAINABLE BY THE ACID-HAEMATEIN TEST FOR PHOSPHOLIPIDS ACCORDING TO BAKER. Z Zellforsch Mikrosk Anat. 1963 Nov 21;61:317-37. PubMed PMID: 14104678.

8: Bronzato M, Zoleo A, Biondi B, Centeno SA. An insight into the metal coordination and spectroscopic properties of artistic Fe and Fe/Cu logwood inks. Spectrochim Acta A Mol Biomol Spectrosc. 2016 Jan 15;153:522-9. doi: 10.1016/j.saa.2015.08.042. Epub 2015 Sep 3. PubMed PMID: 26414555.

9: Wu Y, Tang L, Huang L, Han Z, Wang J, Pan H. A low detection limit penicillin biosensor based on single graphene nanosheets preadsorbed with hematein/ionic liquids/penicillinase. Mater Sci Eng C Mater Biol Appl. 2014 Jun 1;39:92-9. doi: 10.1016/j.msec.2014.02.012. Epub 2014 Feb 17. PubMed PMID: 24863203.

10: Dapson RW, Bain CL. Brazilwood, sappanwood, brazilin and the red dye brazilein: from textile dyeing and folk medicine to biological staining and musical instruments. Biotech Histochem. 2015;90(6):401-23. doi: 10.3109/10520295.2015.1021381. Epub 2015 Apr 20. Review. PubMed PMID: 25893688.

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12: Commandeur F, Acosta O, Simon A, Mathieu R, Fautrel A, Gnep K, Haigron P, de Crevoisier R. Prostate whole-mount histology reconstruction and registration to MRI for correlating in-vivo observations with biological findings. Conf Proc IEEE Eng Med Biol Soc. 2015;2015:2399-402. doi: 10.1109/EMBC.2015.7318877. PubMed PMID: 26736777.

13: Hung MS, Xu Z, Chen Y, Smith E, Mao JH, Hsieh D, Lin YC, Yang CT, Jablons DM, You L. Hematein, a casein kinase II inhibitor, inhibits lung cancer tumor growth in a murine xenograft model. Int J Oncol. 2013 Nov;43(5):1517-22. doi: 10.3892/ijo.2013.2087. Epub 2013 Sep 4. PubMed PMID: 24008396; PubMed Central PMCID: PMC3823374.

14: Smith AA. Hematein chelates of unusual metal ions for tinctorial histochemistry. Biotech Histochem. 2010 Feb;85(1):43-54. doi: 10.3109/10520290903048392. PubMed PMID: 19575317.

15: Kiernan JA, Horobin RW. A special issue devoted to hematoxylin, hematein, and hemalum. Biotech Histochem. 2010 Feb;85(1):5-6. doi: 10.3109/10520290903048368. PubMed PMID: 19548134.

16: Chen B, Ma M, Su X. An amperometric penicillin biosensor with enhanced sensitivity based on co-immobilization of carbon nanotubes, hematein, and beta-lactamase on glassy carbon electrode. Anal Chim Acta. 2010 Jul 26;674(1):89-95. doi: 10.1016/j.aca.2010.06.014. Epub 2010 Jun 16. PubMed PMID: 20638504.

17: Cooksey C. Hematoxylin and related compounds--an annotated bibliography concerning their origin, properties, chemistry, and certain applications. Biotech Histochem. 2010 Feb;85(1):65-82. doi: 10.3109/10520290903048418. PubMed PMID: 19568968.

18: Hung MS, Xu Z, Lin YC, Mao JH, Yang CT, Chang PJ, Jablons DM, You L. Identification of hematein as a novel inhibitor of protein kinase CK2 from a natural product library. BMC Cancer. 2009 May 6;9:135. doi: 10.1186/1471-2407-9-135. PubMed PMID: 19419583; PubMed Central PMCID: PMC2696466.

19: Carpentier P, Foquin A, Dorandeu F. A new use for an old method: the Woelcke myelin stain for counting degenerating neurons in the brain of mice following status epilepticus. Neurotoxicology. 2012 Aug;33(4):789-95. doi: 10.1016/j.neuro.2011.11.007. Epub 2011 Dec 3. PubMed PMID: 22155333.

20: Marshall PN. Papanicolaou staining--a review. Microsc Acta. 1983 May;87(3):233-43. Review. PubMed PMID: 6195507.