Edrophonium Chloride
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MedKoo CAT#: 329002

CAS#: 116-38-1

Description: Edrophonium Chloride, also known as Tensilon, is an acetylcholinesterase inhibitor used in cardiac arrhythmias and in the diagnosis of myasthenia gravis. Edrophonium Chloride has also been used as an antidote to curare principles.


Chemical Structure

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Edrophonium Chloride
CAS# 116-38-1

Theoretical Analysis

MedKoo Cat#: 329002
Name: Edrophonium Chloride
CAS#: 116-38-1
Chemical Formula: C10H16ClNO
Exact Mass: 201.092
Molecular Weight: 201.69
Elemental Analysis: C, 59.55; H, 8.00; Cl, 17.58; N, 6.94; O, 7.93

Price and Availability

Size Price Availability Quantity
100.0mg USD 210.0 2 weeks
250.0mg USD 340.0 2 weeks
500.0mg USD 535.0 2 weeks
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Synonym: Edrophonium Chloride; Tensilon; Tensilon chloride; Antirex; Enlon; Reversol

IUPAC/Chemical Name: N-ethyl-3-hydroxy-N,N-dimethylbenzenaminium chloride

InChi Key: BXKDSDJJOVIHMX-UHFFFAOYSA-N

InChi Code: InChI=1S/C10H15NO.ClH/c1-4-11(2,3)9-6-5-7-10(12)8-9;/h5-8H,4H2,1-3H3;1H

SMILES Code: C[N+](C)(CC)C1=CC=CC(O)=C1.[Cl-]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 201.69 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Matsumoto S, Murakami N, Koizumi H, Takahashi M, Izumi Y, Kaji R. Edrophonium Challenge Test for Blepharospasm. Front Neurosci. 2016 Jun 6;10:226. doi: 10.3389/fnins.2016.00226. eCollection 2016. PubMed PMID: 27375406; PubMed Central PMCID: PMC4894005.

2: Kang H, Takahashi Y, Iwaki M, Asamura S, Kakizaki H. Upper eyelid retraction disclosed after edrophonium chloride administration in a patient with Graves' orbitopathy and myasthenia gravis. Clin Ophthalmol. 2012;6:807-10. doi: 10.2147/OPTH.S29408. Epub 2012 May 28. PubMed PMID: 22701081; PubMed Central PMCID: PMC3373229.

3: Lee CA, Reynolds JC, Ouyang A, Baker L, Cohen S. Esophageal chest pain. Value of high-dose provocative testing with edrophonium chloride in patients with normal esophageal manometries. Dig Dis Sci. 1987 Jul;32(7):682-8. PubMed PMID: 3595381.

4: Gustafsson U, Tibbling L. The effect of edrophonium chloride-induced chest pain on esophageal blood flow and motility. Scand J Gastroenterol. 1997 Feb;32(2):104-7. PubMed PMID: 9051868.

5: Bassotti G, Imbimbo BP, Betti C, Erbella GS, Pelli MA, Morelli A. Edrophonium chloride for testing colonic contractile activity in man. Acta Physiol Scand. 1991 Mar;141(3):289-93. PubMed PMID: 1858502.

6: Yossepowitch O, Gillon G, Baniel J, Engelstein D, Livne PM. The effect of cholinergic enhancement during filling cystometry: can edrophonium chloride be used as a provocative test for overactive bladder? J Urol. 2001 May;165(5):1441-5. PubMed PMID: 11342893.

7: Cooper JA, Baratta F, Frieden J, Grossman J. Hemodynamic effects of edrophonium chloride (Tensilon) infusion. Chest. 1975 Feb;67(2):147-51. PubMed PMID: 1116389.

8: Draczkowski P, Tomaszuk A, Halczuk P, Strzemski M, Matosiuk D, Jozwiak K. Determination of affinity and efficacy of acetylcholinesterase inhibitors using isothermal titration calorimetry. Biochim Biophys Acta. 2016 May;1860(5):967-74. doi: 10.1016/j.bbagen.2015.11.006. Epub 2015 Nov 14. PubMed PMID: 26586471.

9: Hamill TW. Determination of edrophonium chloride injection USP in the presence of preservatives. J Assoc Off Anal Chem. 1979 Sep;62(5):1110-2. PubMed PMID: 528457.

10: Domanovits H, Wenger S, Schillinger M, Mayr N, Holzer M, Laggner AN, Zeitlhofer J. [Edrophonium chloride (Tensilon) test: a safe method in diagnosing myasthenia gravis]. Wien Klin Wochenschr. 2000 Jul 7;112(13):592-5. German. PubMed PMID: 10944818.

11: Magyar P, Szathmáry I, Szobor A. Myasthenia gravis: lung-function studies without and with edrophonium chloride. Eur Neurol. 1979;18(1):59-65. PubMed PMID: 436864.

12: Rossen RM, Krikorian J, Hancock EW. Ventricular asystole after edrophonium chloride administration. JAMA. 1976 Mar 8;235(10):1041-2. PubMed PMID: 765513.

13: Siatkowski RM, Shah L, Feuer WJ. The effect of edrophonium chloride on muscle balance in normal subjects and those with nonmyasthenic strabismus. J Neuroophthalmol. 1997 Mar;17(1):7-11. PubMed PMID: 9093954.

14: Szathmáry I, Magyar P, Szobor A. Myasthenia gravis: protective effect of ipratropiumbromide (Atrovent) on airways obstruction caused by edrophonium chloride (Tensilon). Eur Neurol. 1981;20(1):56-61. PubMed PMID: 6451430.

15: KLEE A, JACOBSEN B. Side effects of the edrophonium chloride (Tensilon) test. Report of an unusual case. Psychiatr Neurol (Basel). 1962;143:306-14. PubMed PMID: 14456697.