WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 526868

CAS#: 936622-80-9 (free base)

Description: HT-61, also known as HY-50A, is a pyrroloquinolone antibiotic potentially for the treatment of staphylococcal infections. HT61 was effective at reducing biofilm viability and was associated with increased expression of cell wall stress and division proteins, confirming its potential as a treatment for S. aureus biofilm infections. HT61 enhances the effect of tobramycin against Pseudomonas aeruginosa in vitro and in vivo.

Chemical Structure

CAS# 936622-80-9 (free base)

Theoretical Analysis

MedKoo Cat#: 526868
Name: HT-61
CAS#: 936622-80-9 (free base)
Chemical Formula: C26H24N2O
Exact Mass: 380.1889
Molecular Weight: 380.491
Elemental Analysis: C, 82.07; H, 6.36; N, 7.36; O, 4.20

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5.0mg USD 150.0 Ready to ship
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Related CAS #: 936622-80-9 (free base)    

Synonym: HT-61; HT61; HT 61; HY-50A; HY 50A; HY50A.

IUPAC/Chemical Name: 4-methyl-1-phenethyl-8-phenoxy-2,3-dihydro-1H-pyrrolo[3,2-c]quinoline


InChi Code: InChI=1S/C26H24N2O/c1-19-23-15-17-28(16-14-20-8-4-2-5-9-20)26(23)24-18-22(12-13-25(24)27-19)29-21-10-6-3-7-11-21/h2-13,18H,14-17H2,1H3


Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

Preparing Stock Solutions

The following data is based on the product molecular weight 380.491 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL

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1: Frapwell CJ, Skipp PJ, Howlin RP, Angus EM, Hu Y, Coates ARM, Allan RN, Webb JS. Antimicrobial Activity of the Quinoline Derivative HT61 against Staphylococcus aureus Biofilms. Antimicrob Agents Chemother. 2020 Apr 21;64(5):e02073-19. doi: 10.1128/AAC.02073-19. PMID: 32122902; PMCID: PMC7179629.

2: Amison RT, Faure ME, O'Shaughnessy BG, Bruce KD, Hu Y, Coates A, Page CP. The small quinolone derived compound HT61 enhances the effect of tobramycin against Pseudomonas aeruginosa in vitro and in vivo. Pulm Pharmacol Ther. 2020 Apr;61:101884. doi: 10.1016/j.pupt.2019.101884. Epub 2019 Dec 27. PMID: 31887372.

3: Hubbard AT, Coates AR, Harvey RD. Comparing the action of HT61 and chlorhexidine on natural and model Staphylococcus aureus membranes. J Antibiot (Tokyo). 2017 Oct;70(10):1020-1025. doi: 10.1038/ja.2017.90. Epub 2017 Aug 2. PMID: 28765589.

4: Hubbard AT, Barker R, Rehal R, Vandera KA, Harvey RD, Coates AR. Mechanism of Action of a Membrane-Active Quinoline-Based Antimicrobial on Natural and Model Bacterial Membranes. Biochemistry. 2017 Feb 28;56(8):1163-1174. doi: 10.1021/acs.biochem.6b01135. Epub 2017 Feb 13. PMID: 28156093.

5: Hu Y, Coates AR. Enhancement by novel anti-methicillin-resistant Staphylococcus aureus compound HT61 of the activity of neomycin, gentamicin, mupirocin and chlorhexidine: in vitro and in vivo studies. J Antimicrob Chemother. 2013 Feb;68(2):374-84. doi: 10.1093/jac/dks384. Epub 2012 Oct 4. PMID: 23042813.

6: Hu Y, Shamaei-Tousi A, Liu Y, Coates A. A new approach for the discovery of antibiotics by targeting non-multiplying bacteria: a novel topical antibiotic for staphylococcal infections. PLoS One. 2010 Jul 27;5(7):e11818. doi: 10.1371/journal.pone.0011818. PMID: 20676403; PMCID: PMC2910736.

7: Ikushima M, Matsui K, Yamada F, Kawahashi S, Nishikawa SK. Specific immune response to a synthetic peptide derived from outer surface protein C of Borrelia burgdorferi predicts protective borreliacidal antibodies. FEMS Immunol Med Microbiol. 2000 Sep;29(1):15-21. doi: 10.1111/j.1574-695X.2000.tb01499.x. PMID: 10967255.


5.0mg / USD 150.0