S31-201 | CAS#501919-59-1 | STAT3 inhibitor

S31-201
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WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 202541

CAS#: 501919-59-1

Description: S3I-201, also known as NSC 74859, is a cell-permeable Stat3 inhibitor that binds to the Stat3-SH2 domain, prevents Stat3 phosphorylation/activation, dimerization, and DNA-binding.

Chemical Structure

S31-201

CAS# 501919-59-1

Product data Instruction

Theoretical Analysis

MedKoo Cat#: 202541
Name: S31-201
CAS#: 501919-59-1
Chemical Formula: C16H15NO7S
Exact Mass: 365.06
Molecular Weight: 365.360
Elemental Analysis: C, 52.60; H, 4.14; N, 3.83; O, 30.65; S, 8.78

Price and Availability

Size	Price	Availability
10mg	USD 90	Ready to ship
25mg	USD 150	Ready to ship
50mg	USD 250	Ready to ship
100mg	USD 450	Ready to ship
200mg	USD 750	Ready to ship
500mg	USD 1450	Ready to ship
Bulk inquiry Welcome, Customer: Please do not inquire quote if your intended use is for a patient since our products are for research use and for chemical synthesis use, not for human use . For in-stock products, we listed price in the web page. You may inquire prices for which sizes were not listed. If no price is listed, this means the product is not in stock at the moment, which may be available via custom synthesis. For cost-effective reason, minimum order of 1g is requested (typically very expensive). The lead time is usually 2-4 months. Quote of less than 1g will not be provided. MedKoo may not offer quote for below reasons: (1) current available synthetic method appears to be extremely expensive which is obviously not affordable to most customers; (2) Key raw material to make the product is temporally not available; (3) DEA controlled substances; (4) the product is under patent protection in customer’s country.

Synonym: NSC 74859; NSC74859; NSC-74859; S3I-201; S3I 20; S3I201.

IUPAC/Chemical Name: 2-hydroxy-4-(2-(tosyloxy)acetamido)benzoic acid

InChi Key: HWNUSGNZBAISFM-UHFFFAOYSA-N

InChi Code: InChI=1S/C16H15NO7S/c1-10-2-5-12(6-3-10)25(22,23)24-9-15(19)17-11-4-7-13(16(20)21)14(18)8-11/h2-8,18H,9H2,1H3,(H,17,19)(H,20,21)

SMILES Code: O=C(O)C1=CC=C(NC(COS(=O)(C2=CC=C(C)C=C2)=O)=O)C=C1O

Appearance: White to off-white solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO and DMF

Shelf Life: >5 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: In HCC cells with loss of response to TGF-.beta., NSC 74859, a STAT3-specific inhibitor, markedly suppresses growth. In contrast, CD133+ status did not affect the response to STAT3 inhibition: both CD133+ Huh-7 cells and CD133- Huh-7 cells are equally sensitive to NSC 74859 treatment and STAT3 inhibition, with an IC50 of 100 .mu.M. Thus, the TGF-.beta./beta2 spectrin (.beta.2SP) pathway may reflect a more functional 'stem/progenitor' state than CD133. Furthermore, NSC 74859 treatment of Huh-7 xenografts in nude mice significantly retarded tumor growth, with an ED of only 5 mg/kg. Moreover, NSC 74859 inhibited tyrosine phosphorylation of STAT3 in HCC cells in vivo. The authors conclude that inhibiting interleukin 6 (IL6)/STAT3 in HCCs with inactivation of the TGF-.beta./.beta.2SP pathway is an effective approach in management of HCCs. Thus, IL6/STAT3, a major signaling pathway in HCC stem cell renewal and proliferation, can provide a novel approach to the treatment of specific HCCs. see: Oncogene (2009), 28(7), 961-972. Current developer : H. Lee Moffitt Cancer Center & Research Institute, USA; University of South Florida; University of Central Florida

Product Data:

Product Data

Certificate of Analysis:

View CoA: current batch, Lot#A8T02K23

QC Data:

View QC data: current batch, Lot#A8T02K23

Safety Data Sheet (SDS):

View Safety Data Sheet (SDS)

Instruction:

View handling instruction

Biological target:	S3I-201 is a Stat3 inhibitor with an IC50 of 86 μM.
In vitro activity:	S3I-201 has the potential to mitigate fibrosis in a cellular context, making it relevant for studying conditions like lupus nephritis. S3I-201 was used in cell culture experiments to suppress the activation of STAT3, which had therapeutic effects by attenuating fibrotic changes in cultured HK-2 cells. Specifically, it reduced the expression of fibrosis-related proteins while improving the expression of E-cadherin. Reference: Int J Biochem Cell Biol. 2021 May;134:105950. https://pubmed.ncbi.nlm.nih.gov/33609746/
In vivo activity:	This study investigated the role of S3I-201 in experimental autoimmune encephalomyelitis (EAE), a model of MS. The severity of clinical scores decreased in S3I-201-treated EAE mice compared to vehicle-treated EAE mice. S3I-201 may have novel therapeutic potential against MS. Reference: Mult Scler Relat Disord. 2023 May;73:104658. https://pubmed.ncbi.nlm.nih.gov/36989705/

Solubility Data

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMF	12.5	34.20
	DMSO	16.0	43.80

Preparing Stock Solutions

The following data is based on the product molecular weight 365.36 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.15 mL	5.76 mL	11.51 mL
5 mM	0.23 mL	1.15 mL	2.3 mL
10 mM	0.12 mL	0.58 mL	1.15 mL
50 mM	0.02 mL	0.12 mL	0.23 mL

Formulation protocol:	1. Yuan C, Ni L, Wu X. Activin A activation drives renal fibrosis through the STAT3 signaling pathway. Int J Biochem Cell Biol. 2021 May;134:105950. doi: 10.1016/j.biocel.2021.105950. Epub 2021 Feb 17. PMID: 33609746. 2. Cheng Z, Lei Z, Yang P, Si A, Xiang D, Tang X, Guo G, Zhou J, Hüser N. Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways. Mol Carcinog. 2019 Aug;58(8):1389-1399. doi: 10.1002/mc.23022. Epub 2019 Apr 17. PMID: 30997702. 3. Ahmad SF, Ansari MA, Nadeem A, Bakheet SA, Al-Mazroua HA, Alomar HA, Al-Hamamah MA, Attia SM. S3I-201, a selective stat3 inhibitor, ameliorates clinical symptoms in a mouse model of experimental autoimmune encephalomyelitis through the regulation of multiple intracellular signalling in Th1, Th17, and treg cells. Mult Scler Relat Disord. 2023 May;73:104658. doi: 10.1016/j.msard.2023.104658. Epub 2023 Mar 23. PMID: 36989705. 4. Du Y, Zhang W, Liu S, Feng X, Gao F, Liu Q. S3I-201 ameliorates tubulointerstitial lesion of the kidneys in MRL/lpr mice. Biochem Biophys Res Commun. 2018 Sep 3;503(1):177-180. doi: 10.1016/j.bbrc.2018.05.207. Epub 2018 Jun 11. PMID: 29885836.
In vitro protocol:	1. Yuan C, Ni L, Wu X. Activin A activation drives renal fibrosis through the STAT3 signaling pathway. Int J Biochem Cell Biol. 2021 May;134:105950. doi: 10.1016/j.biocel.2021.105950. Epub 2021 Feb 17. PMID: 33609746. 2. Cheng Z, Lei Z, Yang P, Si A, Xiang D, Tang X, Guo G, Zhou J, Hüser N. Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways. Mol Carcinog. 2019 Aug;58(8):1389-1399. doi: 10.1002/mc.23022. Epub 2019 Apr 17. PMID: 30997702.
In vivo protocol:	1. Ahmad SF, Ansari MA, Nadeem A, Bakheet SA, Al-Mazroua HA, Alomar HA, Al-Hamamah MA, Attia SM. S3I-201, a selective stat3 inhibitor, ameliorates clinical symptoms in a mouse model of experimental autoimmune encephalomyelitis through the regulation of multiple intracellular signalling in Th1, Th17, and treg cells. Mult Scler Relat Disord. 2023 May;73:104658. doi: 10.1016/j.msard.2023.104658. Epub 2023 Mar 23. PMID: 36989705. 2. Du Y, Zhang W, Liu S, Feng X, Gao F, Liu Q. S3I-201 ameliorates tubulointerstitial lesion of the kidneys in MRL/lpr mice. Biochem Biophys Res Commun. 2018 Sep 3;503(1):177-180. doi: 10.1016/j.bbrc.2018.05.207. Epub 2018 Jun 11. PMID: 29885836.

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1. Pang M, Ma L, Gong R, Tolbert E, Mao H, Ponnusamy M, Chin YE, Yan H, Dworkin LD, Zhuang S. A novel STAT3 inhibitor, S3I-201, attenuates renal interstitial fibroblast activation and interstitial fibrosis in obstructive nephropathy. Kidney Int. 2010 Aug;78(3):257-68. Epub 2010 Jun 2. PubMed PMID: 20520592.

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