CCG1423
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MedKoo CAT#: 406613

CAS#: 285986-88-1

Description: CCG-1423 is a small-molecule inhibitor of RhoA transcriptional signaling. CCG-1423 displays activity in several in vitro cancer cell functional assays. CCG-1423 potently (<1 mumol/L) inhibits lysophosphatidic acid-induced DNA synthesis in PC-3 prostate cancer cells, and whereas it inhibits the growth of RhoC-overexpressing melanoma lines (A375M2 and SK-Mel-147) at nanomolar concentrations, it is less active on related lines (A375 and SK-Mel-28) that express lower levels of Rho.

Chemical Structure

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CCG1423
CAS# 285986-88-1
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 406613
Name: CCG1423
CAS#: 285986-88-1
Chemical Formula: C18H13ClF6N2O3
Exact Mass: 454.05
Molecular Weight: 454.751
Elemental Analysis: C, 47.54; H, 2.88; Cl, 7.80; F, 25.07; N, 6.16; O, 10.55

Price and Availability

Size Price Availability Quantity
10mg USD 90 Ready to ship
25mg USD 150 Ready to ship
50mg USD 225 Ready to ship
100mg USD 385 Ready to ship
200mg USD 685 Ready to ship
500mg USD 1450 Ready to ship
1g USD 2465 Ready to ship
2g USD 4150 Ready to ship
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Synonym: CCG1423; CCG-1423; CCG 1423.

IUPAC/Chemical Name: N-((1-((4-chlorophenyl)amino)-1-oxopropan-2-yl)oxy)-3,5-bis(trifluoromethyl)benzamide

InChi Key: DSMXVSGJIDFLKP-UHFFFAOYSA-N

InChi Code: InChI=1S/C18H13ClF6N2O3/c1-9(15(28)26-14-4-2-13(19)3-5-14)30-27-16(29)10-6-11(17(20,21)22)8-12(7-10)18(23,24)25/h2-9H,1H3,(H,26,28)(H,27,29)

SMILES Code: O=C(NOC(C)C(NC1=CC=C(Cl)C=C1)=O)C2=CC(C(F)(F)F)=CC(C(F)(F)F)=C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:         

Biological target: CCG-1423 is an inhibitor of RhoA/C-mediated gene transcription.
In vitro activity: Fluorescence staining showed that MLK1 inhibition by CCG-1423 (Figure 3B) significantly downregulated ROS (reactive oxygen species) production in F4/80+ macrophages after IRI (ischemia-reperfusion injury). In response to hypoxia-reoxygenation (H/R), there was augmented occupancy of MKL1 on the proximal Nox gene promoters but not on the Gapdh promoters in cultured macrophages (RAW264.7) as demonstrated by ChIP assay (Figure 3C), indicating that MKL1 might directly regulate NOX gene transactivation. Reporter assay confirmed that overexpression of MKL1 dose-dependently activated promoter activities of NOX genes (Figure IVA in the online-only Data Supplement). In contrast, depletion of endogenous MKL1 with a short-hairpin RNA plasmid downregulated NOX promoter activities (Figure IVB). CCG-1423 treatment abrogated the induction of Nox messages (Figure 3D) and proteins (Figure 3E) in a dose-dependent manner. In keeping with reduced Nox expression, there was a decrease in intracellular ROS levels in CCG-1423-treated cells as assayed by both DHE and DCFH-DA stainings (Figure 3F) and quantitative luminescence assay (Figure 3G). Reference: Circulation. 2018 Dec 11;138(24):2820-2836. https://www.ahajournals.org/doi/10.1161/CIRCULATIONAHA.118.035377?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmed&
In vivo activity: The effect of an MKL1 inhibitor CCG-142330 on IRI was investigated in mice. When the mice were injected peritoneally with CCG-1423 for 3 days before the IR procedure, CCG-1423 injection resulted in a significant reduction of infarct size but did not afford detectable improvements in heart function (Figure I). When the mice were injected daily with CCG-1423 for 2 consecutive weeks before the IR procedure and found that prolonged pretreatment with CCG-1423 not only alleviated myocardial infarction (Figure 1E) but mitigated the loss of heart function (Figure 1F through 1H). This discrepancy in the effectiveness of 2 CCG regimens could be partly explained by the observation that although 2 weeks of CCG injection almost completely blocked the nuclear accumulation of MKL1 in cardiac macrophages compared with the vehicle group, 3 days of injection only marginally altered MKL1 localization (Figure II). Taken together, these data suggest that MKL1 loss of function might attenuate myocardial infarction and help retrieve the loss of heart function after IRI. Reference: Circulation. 2018 Dec 11;138(24):2820-2836. https://www.ahajournals.org/doi/10.1161/CIRCULATIONAHA.118.035377?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmed&

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 10.0 21.99
DMSO 42.9 94.27
DMSO:PBS (pH 7.2) (1:5) 0.2 0.33
Ethanol 2.1 4.68

Preparing Stock Solutions

The following data is based on the product molecular weight 454.75 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Zabini D, Granton E, Hu Y, Miranda MZ, Weichelt U, Breuils Bonnet S, Bonnet S, Morrell NW, Connelly KA, Provencher S, Ghanim B, Klepetko W, Olschewski A, Kapus A, Kuebler WM. Loss of SMAD3 Promotes Vascular Remodeling in Pulmonary Arterial Hypertension via MRTF Disinhibition. Am J Respir Crit Care Med. 2018 Jan 15;197(2):244-260. doi: 10.1164/rccm.201702-0386OC. Erratum in: Am J Respir Crit Care Med. 2019 Apr 1;199(7):932. PMID: 29095649. 2. Yu L, Yang G, Zhang X, Wang P, Weng X, Yang Y, Li Z, Fang M, Xu Y, Sun A, Ge J. Megakaryocytic Leukemia 1 Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury. Circulation. 2018 Dec 11;138(24):2820-2836. doi: 10.1161/CIRCULATIONAHA.118.035377. PMID: 30018168.
In vitro protocol: 1. Yu L, Yang G, Zhang X, Wang P, Weng X, Yang Y, Li Z, Fang M, Xu Y, Sun A, Ge J. Megakaryocytic Leukemia 1 Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury. Circulation. 2018 Dec 11;138(24):2820-2836. doi: 10.1161/CIRCULATIONAHA.118.035377. PMID: 30018168.
In vivo protocol: 1. Zabini D, Granton E, Hu Y, Miranda MZ, Weichelt U, Breuils Bonnet S, Bonnet S, Morrell NW, Connelly KA, Provencher S, Ghanim B, Klepetko W, Olschewski A, Kapus A, Kuebler WM. Loss of SMAD3 Promotes Vascular Remodeling in Pulmonary Arterial Hypertension via MRTF Disinhibition. Am J Respir Crit Care Med. 2018 Jan 15;197(2):244-260. doi: 10.1164/rccm.201702-0386OC. Erratum in: Am J Respir Crit Care Med. 2019 Apr 1;199(7):932. PMID: 29095649. 2. Yu L, Yang G, Zhang X, Wang P, Weng X, Yang Y, Li Z, Fang M, Xu Y, Sun A, Ge J. Megakaryocytic Leukemia 1 Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury. Circulation. 2018 Dec 11;138(24):2820-2836. doi: 10.1161/CIRCULATIONAHA.118.035377. PMID: 30018168.

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1: Hayashi K, Watanabe B, Nakagawa Y, Minami S, Morita T. RPEL proteins are the molecular targets for CCG-1423, an inhibitor of Rho signaling. PLoS One. 2014 Feb 18;9(2):e89016. doi: 10.1371/journal.pone.0089016. eCollection 2014. PubMed PMID: 24558465; PubMed Central PMCID: PMC3928398.

2: Bell JL, Haak AJ, Wade SM, Sun Y, Neubig RR, Larsen SD. Design and synthesis of tag-free photoprobes for the identification of the molecular target for CCG-1423, a novel inhibitor of the Rho/MKL1/SRF signaling pathway. Beilstein J Org Chem. 2013 May 21;9:966-73. doi: 10.3762/bjoc.9.111. Print 2013. PubMed PMID: 23766813; PubMed Central PMCID: PMC3678708.

3: Evelyn CR, Bell JL, Ryu JG, Wade SM, Kocab A, Harzdorf NL, Showalter HD, Neubig RR, Larsen SD. Design, synthesis and prostate cancer cell-based studies of analogs of the Rho/MKL1 transcriptional pathway inhibitor, CCG-1423. Bioorg Med Chem Lett. 2010 Jan 15;20(2):665-72. doi: 10.1016/j.bmcl.2009.11.056. Epub 2009 Nov 18. PubMed PMID: 19963382; PubMed Central PMCID: PMC2818594.

4: Evelyn CR, Wade SM, Wang Q, Wu M, Iñiguez-Lluhí JA, Merajver SD, Neubig RR. CCG-1423: a small-molecule inhibitor of RhoA transcriptional signaling. Mol Cancer Ther. 2007 Aug;6(8):2249-60. PubMed PMID: 17699722.