Lys-ala-NH-MEC

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MedKoo CAT#: 530711

CAS#: 94149-28-7

Description: Lys-ala-NH-mec, also known as 7-Lysylalanyl-4-methylcoumarinamide, is a Fluorogenic substrate for dipeptidyl aminopeptidase.

Chemical Structure

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Lys-ala-NH-MEC
CAS# 94149-28-7
Instruction

Theoretical Analysis

MedKoo Cat#: 530711
Name: Lys-ala-NH-MEC
CAS#: 94149-28-7
Chemical Formula: C19H26N4O4
Exact Mass: 374.20
Molecular Weight: 374.441
Elemental Analysis: C, 60.95; H, 7.00; N, 14.96; O, 17.09

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @medkoo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: 7-Lysylalanyl-4-methylcoumarinamide; 7-Lys-ala-4-mca; Lys-ala-NH-mec;

IUPAC/Chemical Name: L-Alaninamide, L-lysyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-

InChi Key: VSRJZLOPKBZWMF-WFASDCNBSA-N

InChi Code: InChI=1S/C19H26N4O4/c1-11-9-17(24)27-16-10-13(6-7-14(11)16)23(12(2)18(22)25)19(26)15(21)5-3-4-8-20/h6-7,9-10,12,15H,3-5,8,20-21H2,1-2H3,(H2,22,25)/t12-,15-/m0/s1

SMILES Code: C[C@@H](C(N)=O)N(C([C@H](CCCCN)N)=O)C1=CC=C(C(O2)=C1)C(C)=CC2=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 374.44 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Chung J, Ulyanov NB, Guilbert C, Mujeeb A, James TL. Binding characteristics of small molecules that mimic nucleocapsid protein-induced maturation of stem-loop 1 of HIV-1 RNA. Biochemistry. 2010 Aug 3;49(30):6341-51. doi: 10.1021/bi100660r. PubMed PMID: 20565056; PubMed Central PMCID: PMC2921804.

2: Smid JR, Young WG, Monsour PA. Dipeptidyl-peptidase II and cathepsin B activities in amelogenesis of the rat incisor. Eur J Oral Sci. 2001 Aug;109(4):260-6. PubMed PMID: 11531072.

3: Cox SW, Gazi MI, Eley BM. Dipeptidyl peptidase II- and IV-like activities in gingival tissue and crevicular fluid from human periodontitis lesions. Arch Oral Biol. 1992 Mar;37(3):167-73. PubMed PMID: 1350193.

4: Smyth M, O'Connor B, O'Cuinn G. Subcellular distribution of dipeptidyl aminopeptidase activity against Lys-Ala-NH-Mec in guinea-pig brain. Biochem Soc Trans. 1991 Feb;19(1):37S. PubMed PMID: 2037170.

5: Nagatsu T, Sakai T, Kojima K, Araki E, Sakakibara S, Fukasawa K, Harada M. A sensitive and specific assay for dipeptidyl-aminopeptidase II in serum and tissues by liquid chromatography-fluorometry. Anal Biochem. 1985 May 15;147(1):80-5. PubMed PMID: 3896025.