BTZO-1
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MedKoo CAT#: 564294

CAS#: 99420-15-2

Description: BTZO-1 is an activator of the glutathione S-transferase Ya subunit (GST Ya) gene ARE by interacting with MIF.

Chemical Structure

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BTZO-1
CAS# 99420-15-2
Product data Instruction

Theoretical Analysis

MedKoo Cat#: 564294
Name: BTZO-1
CAS#: 99420-15-2
Chemical Formula: C13H8N2OS
Exact Mass: 240.04
Molecular Weight: 240.280
Elemental Analysis: C, 64.98; H, 3.36; N, 11.66; O, 6.66; S, 13.34

Price and Availability

Size Price Availability Quantity
10mg USD 250 2 weeks
25mg USD 450 2 weeks
50mg USD 800 2 Weeks
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Synonym: BTZO1; BTZO 1; BTZO-1

IUPAC/Chemical Name: 2-Pyridin-2-yl-1,3-benzothiazin-4-one

InChi Key: GBAKVEWPYUIGHN-UHFFFAOYSA-N

InChi Code: InChI=1S/C13H8N2OS/c16-12-9-5-1-2-7-11(9)17-13(15-12)10-6-3-4-8-14-10/h1-8H

SMILES Code: O=C1N=C(C2=NC=CC=C2)SC3=CC=CC=C13

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.03.00

More Info:

Product Data:
Product Data
Instruction:
View handling instruction
Biological target: BTZO-1 binds to Macrophage migration inhibitory factor (MIF) with a Kd value of 68.6 nM, and its binding requires the N-terminal Pro1.
In vitro activity: BTZO-1 binds to MIF with a K(d) of 68.6 nM, and its binding required the intact N-terminal Pro1. MIF, in the presence of BTZO-1, activated the glutathione S-transferase Ya subunit (GST Ya) gene ARE, whereas reduction of cellular MIF protein levels by siRNA suppressed BTZO-1-induced GST Ya expression. These results suggest that BTZO-1 activates the GST Ya gene ARE by interacting with MIF. Reference: Chem Biol. 2010 Dec 22;17(12):1282-94. https://pubmed.ncbi.nlm.nih.gov/21168764/
In vivo activity: Addition of BTZO-1 during IVM of bovine oocytes improved their developmental competence in the term of improvement of blastocyst rates. Reference: Reprod Domest Anim. 2020 Aug;55(8):1021-1024. https://pubmed.ncbi.nlm.nih.gov/32473610/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.7 32.09
Ethanol 4.0 16.65

Preparing Stock Solutions

The following data is based on the product molecular weight 240.28 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Kimura H, Sato Y, Tajima Y, Suzuki H, Yukitake H, Imaeda T, Kajino M, Oki H, Takizawa M, Tanida S. BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression. Chem Biol. 2010 Dec 22;17(12):1282-94. doi: 10.1016/j.chembiol.2010.10.011. PMID: 21168764. 2. Aboelenain M, El Domany WB, Eldoumani H, Khalil WA. Antioxidant response element activator, BTZO-1, improves the developmental competence of bovine oocytes. Reprod Domest Anim. 2020 Aug;55(8):1021-1024. doi: 10.1111/rda.13733. Epub 2020 Jun 12. PMID: 32473610.
In vitro protocol: 1. Kimura H, Sato Y, Tajima Y, Suzuki H, Yukitake H, Imaeda T, Kajino M, Oki H, Takizawa M, Tanida S. BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression. Chem Biol. 2010 Dec 22;17(12):1282-94. doi: 10.1016/j.chembiol.2010.10.011. PMID: 21168764.
In vivo protocol: 1. Aboelenain M, El Domany WB, Eldoumani H, Khalil WA. Antioxidant response element activator, BTZO-1, improves the developmental competence of bovine oocytes. Reprod Domest Anim. 2020 Aug;55(8):1021-1024. doi: 10.1111/rda.13733. Epub 2020 Jun 12. PMID: 32473610.

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1: Kimura H, Sato Y, Tajima Y, Suzuki H, Yukitake H, Imaeda T, Kajino M, Oki H, Takizawa M, Tanida S. BTZO-1, a cardioprotective agent, reveals that macrophage migration inhibitory factor regulates ARE-mediated gene expression. Chem Biol. 2010 Dec 22;17(12):1282-94. doi: 10.1016/j.chembiol.2010.10.011. PubMed PMID: 21168764.

2: Yukitake H, Takizawa M, Kimura H. Macrophage Migration Inhibitory Factor as an Emerging Drug Target to Regulate Antioxidant Response Element System. Oxid Med Cell Longev. 2017;2017:8584930. doi: 10.1155/2017/8584930. Epub 2017 Jan 16. Review. PubMed PMID: 28191280; PubMed Central PMCID: PMC5278225.