RS-1
featured

    WARNING: This product is for research use only, not for human or veterinary use.

MedKoo CAT#: 406955

CAS#: 312756-74-4

Description: RS-1, also known as RAD51-Stimulatory Compound-1, a RAD51 activator with potential anticancer activity. RAD51 is the central protein that catalyzes DNA repair via homologous recombination, a process that ensures genomic stability. RAD51 protein is commonly expressed at high levels in cancer cells relative to their noncancerous precursors. High levels of RAD51 expression can lead to the formation of genotoxic RAD51 protein complexes on undamaged chromatin.


Chemical Structure

img
RS-1
CAS# 312756-74-4

Theoretical Analysis

MedKoo Cat#: 406955
Name: RS-1
CAS#: 312756-74-4
Chemical Formula: C20H16Br2N2O3S
Exact Mass: 521.92
Molecular Weight: 524.230
Elemental Analysis: C, 45.82; H, 3.08; Br, 30.48; N, 5.34; O, 9.16; S, 6.12

Price and Availability

Size Price Availability Quantity
5g USD -1
100mg USD 650
200mg USD 950
500mg USD 1450
1g USD 2250
2g USD 3850
Bulk inquiry

Synonym: RS-1; RS 1; RS1. RAD51-Stimulatory Compound-1.

IUPAC/Chemical Name: 4-Bromo-N-(4-bromophenyl)-3-[[(phenylmethyl)amino]sulfonyl]benzamide

InChi Key: SWKAVEUTKGKHSR-UHFFFAOYSA-N

InChi Code: InChI=1S/C20H16Br2N2O3S/c21-16-7-9-17(10-8-16)24-20(25)15-6-11-18(22)19(12-15)28(26,27)23-13-14-4-2-1-3-5-14/h1-12,23H,13H2,(H,24,25)

SMILES Code: O=C(NC1=CC=C(Br)C=C1)C2=CC=C(Br)C(S(=O)(NCC3=CC=CC=C3)=O)=C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO and DMF

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Product Data:
Biological target: RS-1 is an activator of DNA repair protein RAD51 (Kd = 48-107 for human RAD51 with different cofactors present).
In vitro activity: RS-1 can increase both protein-DNA complex lengths and the pitch of helical filament turns. RS-1 stimulated hRAD51-mediated homologous strand assimilation (D-loop) activity by at least 5- to 11-fold, depending on the condition. RS-1 enhances the homologous recombination activity of hRAD51 by promoting the formation of active presynaptic filaments. RS-1 can enhance filament stability. Reference: Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15848-53. https://pubmed.ncbi.nlm.nih.gov/18840682/
In vivo activity: This study found that the RS-1 significantly increased knock-in efficiency in vitro, with up to a five-fold improvement at different sites. In animal production, RS-1 yielded substantial improvements. This study provides insights for in vivo nuclease-mediated knock-in applications, enhancing gene-targeting efficiencies in pluripotent stem cells. Reference: Nat Commun. 2016 Jan 28;7:10548. https://pubmed.ncbi.nlm.nih.gov/26817820/

Solubility Data

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMF 30.0 57.23
DMSO 20.0 38.15

Preparing Stock Solutions

The following data is based on the product molecular weight 524.23 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol: 1. Jayathilaka K, Sheridan SD, Bold TD, Bochenska K, Logan HL, Weichselbaum RR, Bishop DK, Connell PP. A chemical compound that stimulates the human homologous recombination protein RAD51. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15848-53. doi: 10.1073/pnas.0808046105. Epub 2008 Oct 7. PMID: 18840682; PMCID: PMC2572930. 2. Bi R, Li Y, Xu M, Zheng Q, Zhang DF, Li X, Ma G, Xiang B, Zhu X, Zhao H, Huang X, Zheng P, Yao YG. Direct evidence of CRISPR-Cas9-mediated mitochondrial genome editing. Innovation (Camb). 2022 Sep 27;3(6):100329. doi: 10.1016/j.xinn.2022.100329. PMID: 36275864; PMCID: PMC9579715. 3. Song J, Yang D, Xu J, Zhu T, Chen YE, Zhang J. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016 Jan 28;7:10548. doi: 10.1038/ncomms10548. PMID: 26817820; PMCID: PMC4738357.
In vitro protocol: 1. Jayathilaka K, Sheridan SD, Bold TD, Bochenska K, Logan HL, Weichselbaum RR, Bishop DK, Connell PP. A chemical compound that stimulates the human homologous recombination protein RAD51. Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15848-53. doi: 10.1073/pnas.0808046105. Epub 2008 Oct 7. PMID: 18840682; PMCID: PMC2572930. 2. Bi R, Li Y, Xu M, Zheng Q, Zhang DF, Li X, Ma G, Xiang B, Zhu X, Zhao H, Huang X, Zheng P, Yao YG. Direct evidence of CRISPR-Cas9-mediated mitochondrial genome editing. Innovation (Camb). 2022 Sep 27;3(6):100329. doi: 10.1016/j.xinn.2022.100329. PMID: 36275864; PMCID: PMC9579715.
In vivo protocol: 1. Song J, Yang D, Xu J, Zhu T, Chen YE, Zhang J. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016 Jan 28;7:10548. doi: 10.1038/ncomms10548. PMID: 26817820; PMCID: PMC4738357.

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Mason JM, Logan HL, Budke B, Wu M, Pawlowski M, Weichselbaum RR, Kozikowski
AP, Bishop DK, Connell PP. The RAD51-stimulatory compound RS-1 can exploit the
RAD51 overexpression that exists in cancer cells and tumors. Cancer Res. 2014 Jul
1;74(13):3546-55. doi: 10.1158/0008-5472.CAN-13-3220. PubMed PMID: 24753542;
PubMed Central PMCID: PMC4079730.